Skip to main content
. 2012 Dec 12;288(6):4148–4157. doi: 10.1074/jbc.M112.436741

FIGURE 6.

FIGURE 6.

Retention of SRY in the nucleus is independent of hsc70. A and B, representative CLSM images of recovery of nuclear fluorescence after photobleaching in HeLa cells transiently transfected to express full-length GFP-SRY-wild type (WT; A) and GFP-SRY-R133W (B) treated with or without siRNA to hsc70 over time (s), after photobleaching (post-bleach). The yellow dotted line indicates the bleached area (∼50% of nucleus). C, quantification of the recovery over time of nuclear fluorescence after photobleaching (Fnbleach, expressed in terms of fractional recovery of respective time points relative to the Fnnon-bleach prebleach value). Results shown are for a representative cell. D, pooled data for the mean ± S.E. (n = 3) percentage maximal recovery of Fnbleach, normalized to 0 s post-bleach.