TABLE 1.

Pooled data for nuclear transport kinetics in vivo as determined in FRAP experiments

Pooled data (mean ± S.E.) from assays performed as described in Fig. 5. Fract. recovery is the maximal recovery of fluorescence, Init. Rate is the initial rate of recovery (Fn/c), and t_½ indicates the time taken to reach half maximal fluorescence recovery.

Sample	Kinetic parameters for nuclear transport
	Fract. recovery	Init. rate	t½	n
		Fn/c s−1	s
GFP-SRY control	0.63 ± 0.05	0.10 ± 0.02	36.8 ± 4.5	23
GFP-SRY + siRNA hsc70	0.48 ± 0.05a	0.05 ± 0.01a	66.2 ± 15.0a	18
GFP-T-ag control	0.40 ± 0.03	0.009 ± 0.002	47.0 ± 7.9	22
GFP-T-ag + siRNA hsc70	0.41 ± 0.02	0.007 ± 0.001	57.2 ± 6.8	28

^a p < 0.05 for significant differences compared with control (Student's t test, performed using GraphPad Prism software (version 5)).