Skip to main content
. 2012 Dec 18;288(6):4158–4173. doi: 10.1074/jbc.M112.434183

FIGURE 1.

FIGURE 1.

mtPE synthesis in Pisd KD cells and PSB-2 cells. A, quantitative PCR analysis of Pisd mRNA relative to cyclophilin mRNA in CHO cells transiently transfected with DsiRNAs targeted to distinct regions of hamster Pisd (KD#1, KD#2, and KD#3) or with nontargeting DsiRNA (NegCtrl); Cy5-KD#1 = fluorophore-labeled KD#1. Data are means ± S.D. of triplicate analyses from two independent transfections (*, p < 0.05 versus NegCtrl). B, PSD activity in Pisd KD#1, KD#2, KD#3, and NegCtrl cells 48 and 72 h after transfection. Data are means ± S.D. from triplicate analyses of two independent experiments (*, p < 0.0001 versus NegCtrl). C–F, [3H]serine incorporation into PE and PS in Pisd KD#1 and NegCtrl cells transfected for 72 h (C and D) and PSB-2 and WT cells (E and F). Data are means ± S.D. of triplicate analyses of three independent experiments (p < 0.05 versus control at 2, 4, and 6 h); some error bars are too small to be visible.