FIGURE 4.

EPR spectra from the Ca²⁺ binding loops, the A-B and F-G loops, and the N terminus of the CBD1 domain of CBD12. The solid lines in panels A and B are EPR data collected with no bound Ca²⁺, and the superimposed dashed lines are data collected in the presence of saturating level of Ca²⁺. The data in panel C were collected from wt-CBD12 in the presence of 10 mm Ca²⁺ (left lane) after EDTA treatment (center lane) and after the addition of 10 mm Ca²⁺ to the EDTA-treated sample (right lane).