FIGURE 5.

Overexpression of TMF fragments disrupts glycosylation. A, Hela cells transfected with an inert plasmid (Mock) or with GFP-tagged full-length (full), central coil, Cterm or Nterm constructs of TMF were stained with GNL-FITC (left column) and DAPI (right column) 72 h after transfection. Transfection efficiency was 50–80% as verified prior to the methanol fixation that destroys GFP fluorescence. Four separate fields of view (two for mock) are shown for each condition. Scale bar 200 μm. B, quantification of the images in A.