Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2012 Dec 4;288(6):4241–4250. doi: 10.1074/jbc.M112.440396

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2013 by The American Society for Biochemistry and Molecular Biology, Inc.

PMC Copyright notice

FIGURE 3. — N-WASP interaction with p120-catenin occurs independent of Arp2. HPAEC transfected with Arp2 (siArp2), N-WASP (siN-WASP), or scrambled (siSc) siRNA were stimulated with thrombin for 30 min, after which the cell lysates were immunoprecipitated using N-WASP or p120-catenin antibodies and immunoblotted with p120-catenin, actin, Arp2, or N-WASP antibodies. A, top panels, representative immunoblot showing the effect of Arp2 knockdown (inset) on N-WASP interaction with p120-catenin and actin. B, top panels, representative immunoblot showing the effect of N-WASP knockdown (inset) on interaction of p120-catenin with Arp2 and actin. A and B, bottom panels, the plot shows mean ± S.D. of -fold increase in interaction between indicated proteins from three individual experiments. * indicates significance from unstimulated siSc transfected cells (p < 0.05). # indicates significance from the scrambled siRNA transfected cells at the respective time point (p < 0.05).