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. 2012 Dec 4;288(6):4241–4250. doi: 10.1074/jbc.M112.440396

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FIGURE 5. — p120-catenin does not mediate N-WASP activation. HPAEC transfected with scrambled (siSc) siRNA or p120-catenin siRNA (sip120-catenin) were stimulated with thrombin for 30 min, after which lysates were immunoprecipitated with N-WASP antibody followed by immunoblotting with the indicated antibodies to determine N-WASP activity. The inset shows an immunoblot of p120-catenin depletion in endothelial cells. Plot shows mean ± S.D. of fold increase in interaction between indicated proteins from three individual experiments. * indicates significance from control unstimulated monolayer within each group (p < 0.05). pTyr, total phospho-Tyr antibody; p-N-WASP, N-WASP antibody.