FIGURE 6.

VCA domain of N-WASP interacts with p120-catenin. Left, COS7 cells were transfected with the indicated constructs. Forty-eight h later, the lysates were immunoprecipitated with anti-GFP antibodies followed by immunoblotting with the indicated antibodies to assess interaction with p120-catenin or Arp2. Right, the top panel shows representative immunoblots, whereas the bottom panel shows the mean ± S.D. of densitometric analysis of interaction between WASP, Arp2, and p120-catenin from three individual experiments. * indicates significance when compared with Y256F, GFP, GFP-N-WASP, and CA (p < 0.05).