FIGURE 4.
Mutation E143A affects MamK filament disassembly. Polymerization of 9.52 μm wild type MamK (A) and MamKE143A (B) protein was assessed by light scattering assays in the presence of 25 and 50 μm and 2 mm ATP-MgCl2. Wild type protein signals decayed with very slow kinetics indicating the lack of dynamic instability. MamKE143A reaches the same maximal light scattering intensity at all ATP-MgCl2 concentrations used and does not exhibit decay in light scattering intensities. Each trace for a particular protein concentration is the average of three measurements on the same day.