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pH dependence of sulfheme formation. A, α-subunit. B, β-subunit. C, AHSP·α-subunit complex. The reactions of Fig. 3 were repeated at pH values 5–8 at 23 °C. Buffers used include 200 mm potassium phosphate (pH 7.0 and 8.0 at 23 °C) and 200 mm sodium acetate (pH 5.0 and 6.0 at 23 °C) (27). Spectra were each offset by a constant absorbance value to aid in visualization.
