FIGURE 6.

LPS-induced mmu-miR-7578 expression was NF-κB pathway-dependent. A, schematic diagram for p65-binding sites upstream of mmu-miR-7578. The arrow represents two predicted sites for p65 binding. B, NF-κB subunit p65 bound with the intron encompassing mmu-miR-7578. TNF-α served as a positive control. Nontarget referred to the amplification of region without the potential NF-κB-binding site that was ∼10 kb downstream of mmu-miR-7578. C, SN50 impaired the induction of mmu-miR-7578 by LPS. SN50M was a mutant control of SN50. Upper panel, Northern blot assay. Lower panel, quantitative RT-PCR of mmu-miR-7578 expression. Data shown represent at least two independent experiments.