FIGURE 7.

mmu-miR-7578 helps protect mice from endotoxin shock in vivo. A, expression of mmu-miR-7578 in small intestine, liver, kidney, and spleen of mmu-miR-7578 transgenic mice compared with their nontransgenic littermates (n = 3 per group). tRNA on the transferred membrane was stained, serving as an internal control. Tg, transgenic mice. Ctrl, nontransgenic littermates. B, survival of transgenic mice subjected to endotoxin shock (n = 10 per group). C, TNFα and IL6 levels in plasma from mmu-miR-7578 transgenic mice (n = 7 per group) or control littermates (n = 6 per group). D, mmu-miR-7578 expression in the LPS-challenged peripheral macrophages isolated from transgenic mice or control littermates. E, proposed model for mmu-miR-7578 inhibition of pro-inflammatory cytokine secretion via Egr1. Data are representative of at least two independent experiments in A and D.