FIGURE 4.

Mucous neck, chief, and pit cell markers in Agr2 WT and Agr2 KO mice. A, shown is Agr2 WT gastric corpus and antrum labeled with anti-AGR2 antisera (green) and the mucous neck cell marker, GSII lectin (red). B and C, shown is double-labeling with anti-TFF2 antisera (green) and GSII lectin (red) of Agr2 WT and Agr2 KO mice in the corpus (B) and antrum (C). D, shown is an area of pronounced antral hyperplasia. Data were obtained from 6-week-old mice (A–C) and from a 16-week-old Agr2 KO mouse (D). E, shown are 3-week-old Agr2 WT and Agr2 KO corpus labeled with anti-gastric intrinsic factor (green) and GSII lectin (red). F, shown is labeling of Agr2 WT and Agr2 KO corpus at 6 and 16 weeks with anti-ATP4A antisera that binds a H⁺:K⁺-ATPase subunit specifically expressed by parietal cells. Nuclei are stained blue with DAPI. All scale bars represent 25 μm.