TABLE 1.
qRT-PCR of stomach RNA
RNA was extracted at 6 and 16 weeks of age from the corpus and antrum of Agr2 WT and Agr2 KO mice. The results are expressed as a ratio of the KO to the WT. − signifies no significant expression from which to assess a ratio.
| Gene | qRT-PCR of RNA from the corpus and antrum (null/WT) |
|||
|---|---|---|---|---|
| 6 Weeks old |
16 Weeks old |
|||
| Corpus | Antrum | Corpus | Antrum | |
| Pitt cell | ||||
| MUC5AC | 1.04 | 1.41 | 0.11 | 0.20 |
| Parietal cell | ||||
| ATP4A | 0.71 | − | 0.049 | − |
| ATP4B | 0.95 | − | 0.042 | − |
| Chief cell | ||||
| PGC | 1.32 | − | 0.33 | − |
| GIF | 0.87 | − | 0.15 | − |
| Mucous neck cell | ||||
| TFF2 | 0.97 | 0.66 | 6.28 | 2.64 |
| Enteroendocrine | ||||
| CHGA | 1.10 | 0.81 | 0.13 | 0.13 |
| CHGB | 1.11 | 0.31 | 0.37 | 0.37 |
| GAST | − | 0.29 | − | 0.84 |
| GHRL | 0.84 | − | 0.32 | − |
| SST | 1.41 | 2.07 | 0.23 | 0.07 |
| ER stress | ||||
| HERPUD1 | 1.05 | 2.10 | 1.46 | 0.65 |
| HSPA5 | 0.98 | 1.62 | 1.80 | 1.15 |
| Growth factors | ||||
| REG1 | 0.54 | 0.54 | 36.76 | 100.43 |
| REG3B | 0.49 | 0.73 | 66.26 | 207.94 |
| REG3G | 0.90 | 0.44 | 71.01 | 247.28 |
| Miscellaneous | ||||
| SOX9 | 1.54 | 1.25 | 3.61 | 1.57 |