FIGURE 5.

Snail increases drug resistance by repressing Bak1 through miR-125b. A and B, HMLE-vector, HMLE-miR-125b, MDA231 scramble, and MDA231-miR-125bspong1 cells were seeded into 96-well plates at a density of 8 × 10³ cells per well and then treated with different concentrations of Taxol or Gemcitabine for 48 h. The inhibition of cell viability was detected. Points represent the mean of three independent experiments; Bars represent S.E. C, HMLE-Snail was transfected with 100 nm anti-neg (Ctr) or anti-miR-125b. Cells were seeded 24 h after transfection into 96-well plates at a density of 5 × 10³ cells per well, incubated for 8 h, and then treated with different concentrations of Taxol. After 48 h of Taxol treatment, inhibition of viability was measured. D, HMLE-Snail cells were transfected with 4 μg of puro3vector and puro3-Bak1. 24 h after transfection, cells were seeded into 96-well plates at the density of 5 × 10³ cells per well, incubated for 8 h, and then treated with different concentrations of Taxol. 48 h after Taxol treatment, inhibition of viability was assessed.