FIGURE 7.

miR-125b and Bak1 are required for Snail-induced breast cancer stem cell enrichment. A, HMLE-Snail-scramble and HMLE-Snail-mir-125b-spong1 cells were incubated with CD24 and CD44 antibodies for 45 min, washed, and then analyzed by flow cytometry. B, HMLE-miR-125b cells were transfected with puro-vector or puro-Bak1. 24 h after transfection, cells were harvested and incubated with CD24 and CD44 for 45 min, washed, and then analyzed by flow cytometry. Cell lysates were prepared for Western blotting. Antibodies against Bak1 and β-actin were used. C, HMLE-Snail cells were transfected with puro-vector or puro-bak1. 24 h after transfection, cells were harvested and were incubated with CD24 and CD44 antibodies for 45 min, washed, and then analyzed by flow cytometry. Non-stem cells represent non-stem cell population. Stem cells represent enriched stem cell population. Columns represent the mean of three independent experiments; bars represent S.E. ***, p < 0.001.