FIGURE 2.

Stacked polypeptide alignment of pyruvate:ferredoxin oxidoreductase primary sequences. Eight PFOR sequences were used for an alignment using ClustalW2 and WebLogo 3 (89, 90). These sequences were C. reinhardtii PFR1 derived from the cDNA obtained in this study, Volvox carteri f. nagariensis (Phytozome v8.0, V. carteri Vocar20008508m), Chlorella variabilis (GenBank^TM EFN55341.1), C. acetobutylicum PFO (GenBank^TM NP_348846.1), Clostridium pasteurianum (GenBank^TM AAD55756.1), Desulfovibrio africanus POR (GenBank^TM CAA70873.1), E. coli YdbK (GenBank^TM YP_002999180.1), and Synechococcus sp. PCC.7002 NifJ (GenBank^TM ACA99434.1). The conserved YPITP substrate-binding site (58), as well as three [4Fe-4S]-cluster coordinating motifs and the region homologous to TPP-binding (59, 91) sites, are underlined. Note that the third [4Fe-4S]-cluster-binding site is atypical and consists of the CXXC motif at positions 942–945 and two separated cysteines at positions 970 and 1221 (60, 61). The first amino acid of recombinant PFR1 is marked by an asterisk.