Constructs used in the present work and their corresponding nomenclatures. Full-length and partial Kir6.2 and split SUR1 fusion constructs are shown as linear representations at the left (dashed segments are deletions from the construct), with nomenclatures listed at the right. N′, N termini; C′, C termini; M1, transmembrane helix 1; M2, transmembrane helix 2; SF, selectivity filter; TMD0, transmembrane domain zero; TMD1, transmembrane domain 1; TMD2, transmembrane domain 2; NBD1, nucleotide-binding domain 1; NBD2 , nucleotide-binding domain 2.