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. 2012 Dec 6;288(6):4378–4388. doi: 10.1074/jbc.M112.388629

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© 2013 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 4. — Kir6.2 constructs with YFP and CFP tagged at N and C termini exhibit FRET. A, apparent FRET efficiencies of singly YFP-tagged and CFP/YFP double-tagged Kir6.2 constructs. These constructs were expressed in COSm6 cells, and apparent FRET efficiencies were measured as the ratio of YFP emission (525 nm) excited by 430 nm to maximum YFP emissions (525 nm) excited by 480-nm light. All of the data are presented as the means ± S.E. with n = 6–10. B, emission spectra (Ex = 430 nm) of COSm6 cell extracts expressing Y6.2C fusion constructs before and after 0.1 mg/ml trypsin digestion. The increase in donor (CFP) emission and the decrease in acceptor (YFP) emission are marked by dashed arrows.