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. 2012 Dec 24;288(6):4389–4404. doi: 10.1074/jbc.M112.400184

FIGURE 3.

FIGURE 3.

Knockdown of Gli2 down-regulates MyoD and MEF2C expression during skeletal myogenesis in P19 cells. P19-shScrambled and P19-shGli2 were differentiated in the presence of 1% DMSO. A, day 9 P19-shScrambled and P19-shGli2 were examined by immunofluorescence for MHC expression. Nuclei were stained with Hoechst dye. Scale bar, 30 μm. B, MHC-positive skeletal myocytes from B were counted in 10 random fields and expressed as % of the total number of nuclei, n = 3. In total, 29,000 cells were counted. n.s., not significant. C, QPCR analysis of the expression of indicated genes in P19-shScrambled and P19-shGli2 cell lines at the times shown. Error bars represent ± S.E. from two pooled colonies for each shRNA construct (two shRNA/cell line) and three biological replicas (n = 6); *, p < 0.05, and **, p < 0.01.