FIGURE 1.

Deletion of efp results in increased retention of polysomes. Shown is the quantification of representative A₂₅₄ profiles of ribosomal fractions isolated from BW25113 (WT) or Δefp, ΔpoxA, or ΔyfcM E. coli strains and separated via sucrose gradient centrifugation. Cultures were grown to mid-log and in some cases (+) exposed to 100 μg/ml chloramphenicol (CAM) for 2 min prior to harvesting. The P/M ratio for each fractionation is calculated from three separate experiments.