FIGURE 2.

Initiation of translation of poxB mRNA in the presence of β-Lys-EF-P. Representative graphs of 50 S and 30 S preinitiation complex joining are shown as light scattering over time. Rates were measured in the presence or absence of unmodified EF-P or β-Lys-EF-P and fit to the single exponential equation (y = m1 × exp(−m0 × m2) + m3 + m4 × m0). k_obs = 0.0088 ± 0.0013 s-1 (BSA), 0.0089 ± 0.0009 s⁻¹ (EF-P), and 0.0082 ± 0.0027 s⁻¹ (βLys-EF-P).