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Elongation of a poly-Phe peptide in the presence of modified EF-P. The rate of poly(U)-directed poly-Phe synthesis by 70 S ribosomes was measured by the incorporation of [14C]Phe. Reactions were performed in the presence or absence of unmodified EF-P (●), β-Lys-EF-P (▴), or BSA (■), preincubated with 70 S ribosomes. Error bars represent the S.D. of three separate experiments.
