FIGURE 6.

Salmonella ΔyfcM mutants do not phenocopy ΔpoxA, ΔyjeK, and Δefp mutants. Salmonella mutants deficient in yfcM exhibit phenotypes similar to that of wild type Salmonella 14028s when grown under hypoosmolar conditions (antibiotic medium 2 agar plates (A)) or when exposed to gentamicin (B), lauryl sulfobetaine (C), or S-nitrosoglutathione (GSNO) (D). ΔyfcM mutants also do not exhibit a migration defect on soft agar plates (E) or a change in 1-N-phenylnaphthylamine accumulation (NPN) (F). Data points represent the average of three independent experiments. Error bars represent S.E. For 1-N-phenylnaphthylamine accumulation, a strain lacking the AcrAB multidrug efflux pump served as a positive control, and standard errors are shown only at 0, 2.5, 5, and 9.5 min for clarity. AU, arbitrary fluorescence units.