FIGURE 5.

MPTP-mediated ROS production in Neuro-2A cells stably expressing CYP2D6. Stable Neuro-2A cells were grown with or without added MPTP (400 μm) and/or CYP2D6-specific inhibitor quinidine (10 μm) or a mitochondrion-specific antioxidant, mito-CP (0.5 μm). A, extracellular H₂O₂ levels were measured using the Amplex Red method as described under “Materials and Methods” in mock, WT2D6, +SRP, and −SRP cells. MPTP treatment (400 μm) was carried out for 48 h. B, effect of superoxide dismutase (SOD) and catalase on ROS in −SRP cells treated with or without MPTP C, effect of different inhibitors/antioxidant on ROS production in −SRP cells. 10 μm quinidine, deprenyl, and pargyline and 0.5 μm of mito-CP were used. MPTP treatment was as in A. Values represent the means ± S.E. of four separate assays.