FIGURE 7.

Effects of MPTP on the differentiation of Neuro-2A cells expressing mitochondrion-targeted CYP2D6. Neuro-2A cells were induced to differentiate for 72 h with 1 mm dibromo-cAMP. In some plates, MPTP (400 μm) was added after 24 h of differentiation. At the end of treatment, cells were fixed in ice-cold methanol, stained with hematoxylin and eosin, and viewed through an Olympus upright microscope. A, effects of MPTP on differentiated state of Neuro-2A cells. Panels i and ii, mock-transfected cells, without and with MPTP, respectively. Panels iii and iv, −SRP CYP2D6-expressing cells treated without and with MPTP, respectively. B, immunoblot of whole cell extracts (50 μg each) with antibody to TH (1:2000 dilution, v/v) (Immuno StAR, Hudson, WI). Blots for cell lysate were co-developed with antibody to actin (1:5000 dilution, v/v; Abcam, Cambridge, MA) as loading control. Panel ii shows quantitation of the immunoblot probed with TH antibody. UD, undifferentiated; Diff, differentiated.