TABLE 1.
Quantification of MPTP metabolites in mitochondria from Neuro-2A cells stably transduced with human CYP2D6 cDNA constructs
Assays were run with mitochondrial proteins from different cell lines, and the metabolites were identified by LC/MS as described under “Materials and Methods” using appropriate internal markers and standard curves for converting peak areas to molar concentration of metabolites.
| Constructs | Nanomoles of metabolite/mg of protein |
||||
|---|---|---|---|---|---|
| MPTP | MPDP | MPP+ | MPTP-OH | PTP | |
| Mock | 70.29 | 0.39 | 0.1 | 0.1 | 0.1 |
| WT2D6 | 94.05 | 3.56 | 0.59 | 0.2 | 0.1 |
| +SRP 2D6 | 89.1 | 0.2 | 0.06 | 0.06 | 0.06 |
| −SRP 2D6 | 113.85 | 7.62 | 1.28 | 0.01 | 0.1 |
| Rat brain mitochondria | 82.1 | 30.69 | 3.56 | 0.29 | 0.08 |