Fig. 2.

Semiautomatic measurement setup for the DAIMM method. (A) The three-axis chamber (computer-aided design; Drawings 7.4, Dassault Systemes) is designed with the electronic circuit in the x-axis, the perfusion circuit in the y-axis, and an optical pathway in the z-axis. Note that the disposable carrier of the single-spike electrode is placed in the cross point of the three axis, and subsequently the chamber is sealed via a transparent glass lid. (B) Carrier blank. (C) The manufacturing process of a carrier with integrated single-spike electrode. A finely etched Ag-bond wire is threaded through the inner tube of the disposable carrier along the x-axis. Adhesive and subsequently ultraviolet light are applied under micromanipulator control using the y-axis. Visual control is given via the z-axis.