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Osteogenic differentiation of the two iHAE cell lines. (A) Alizarin Red S staining of iHAE A and B cells with or without osteogenic induction. (Upper left) iHAE A cells without induction; (upper right) iHAE A cells with induction; (lower left) iHAE B cells without induction; (lower right) iHAE B cells with induction. Scale bar, 200 μm. (B) RT-PCR of osteogenic specific genes (ALP and OPN) from iHAE A and iHAE B cells with (+) or without (−) osteogenic differentiation.
