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. 2013 Feb;15(1):55–67. doi: 10.1089/cell.2012.0021

FIG. 7.

FIG. 7.

Neuronal differentiation of the two iHAE cell lines. (A) Phase-contrast image of iHAE cells with (+) or without (−) neuronal induction. Scale bar, 100 μm. (B) Immunocytochemistry of neuronal specific markers MAP2 (green), GFAP (red), Tuj1 (green), and Nestin (red) with or without induction of neuronal differentiation. All the images are merged with nuclear staining of Hoechst 33342 (blue). Scale bar, 100 μm. (C) RT-PCR of neuronal-specific genes (MAP2, GFAP, TUJ, and CHAT) from iHAE A and iHAE B cells with (+) or without (−) induction of neuronal differentiation.