Fig. 5.

Probing cellular and subcellular details with the multimode fiber endoscope. Images of fluorescently stained neuronal cells acquired with the multimode fiber endoscope and compared against conventional images acquired with a microscope objective. First column, (a) widefield fluorescent image of a single neuron soma and (d) detail of dendrites, middle row; (b) and (e) direct stitched image as acquired from the fiber and right row, image from the fiber resampled and filtered so that the pixelation induced by the scanning acquisition is overcome. Highly detailed images of the neuronal soma and the dendritic network can be resolved by the fiber imaging system. The high quality of the images can make this endoscope useful for diagnostic purposes based on cellular phenotype. The working distance is 200μm to compensate for the coverslip that separates the cells from the fiber facet. Field of view is 60μm by 60μm and scale bars in all images are 10μm.