Figure 2 .

I-SceI-induced reduction. (A) Reduction schematic. At the top is the 80-kb duplication resulting from integration of pWalkman{nbs-Ilp}IS1. The I-SceI endonuclease creates a DSB at IS1. The two nbs-Ilp regions align with three possible positions of recombination (“X”). The repair of the DSB gives rise to four types of events diagrammed below, with the first three being the product of recombination between the duplicated copies. The numbers in circles for the potential recombination sites correspond to the resulting types of reduction products. The positions of PCR primer sets are indicated (half arrows). In the NHEJ-type product, the white gene is disrupted (“broken” oval). The primer positions for “vector PCR” in B are indicated as half arrows. (B) Representative results for diagnostic PCR tests. The PCR templates for the top panels are the following: wt for lanes 1 and 5; a duplicated line for lanes 2 and 6; a type 3 reduction for lanes 3 and 7; a type 1 reduction for lane 4; and a type 2 event for lane 8. The 0.7-kb marker is indicated between the gel pictures. The PCR templates for the bottom panel are a reduction line for lane 9 and different NHEJ-type lines for lanes 10–16. Lane “m” represents markers with size indicated to the left in kilobases.