Table 1 . Frequency of reduction events.
Reduction typee |
||||||
---|---|---|---|---|---|---|
Constructa | Source of DSBb | White-loss frequencyc | Reduction frequencyd | 1 | 2 | 3 |
pWalkman{nbs-Ilp} | ||||||
IS1 | I-SceI | 10% (24/230) | 29% (6/21) | 5 | 1 | 0 |
P transposase | 13% (19/150) | 37% (7/19) | 6 | 1 | 0 | |
Spontaneousf | ||||||
Female | 0.1% (39/35,000) | 37/37 | 1 | 35 | 1 | |
Femaleg | 0.1% (22/15,000) | 8/8 | 1 | 7 | 0 | |
Male | 0.0% (0/8000) | 0 | ||||
IS2 | I-SceI | 13% (45/350) | 39% (13/33) | 6 | 1 | 6 |
Spontaneousf female | 0.1% (11/13,000) | 11/11 | 0 | 11 | 0 |
Constructs used for site-specific integration.
The source of DSB that led to the reported reductions.
For induced events, the frequency was calculated by dividing the number of male parents yielding white-eyed progeny over the total number of males tested. The actual counts are included in parentheses. For spontaneous events, the frequency was calculated by dividing the number of white-eyed offspring over the total number of progeny. The counts are included in parentheses.
Calculated by dividing the number of confirmed reductions over all white-loss events tested by molecular means.
For the molecular structure of different types of reduction events, see Figure 2.
Spontaneous events are categorized according to the gender of the duplication-carrying parents.
Flanking recessive markers were used to demonstrate homolog exchanges (see text).