Table 1 . Captured segment exchange at the tsh locus.
| P[acman] constructa | Insert size (kb) | Engineered mutation | y− w− (exchange)b | Total scored | % exchangedc |
|---|---|---|---|---|---|
| tsh50 #1 | 50 | None | 27 | 132 | 20.5 |
| tsh50 #2 | 50 | None | 10 | 31 | 32.3 |
| tsh1 #1 | 1 | ∼49-kb deletion | 21 | 151 | 13.9 |
| tsh1 #2 | 1 | ∼49-kb deletion | 11 | 132 | 8.3 |
| tsh50ΔNC1 | ∼50 | 312-bp deletion | 8 | 99 | 8.1 |
| tsh50ΔNC2 | ∼50 | 550-bp deletion | 8 | 45 | 17.8 |
Flies were subjected to the schemes outlined in Figures 2 and 4 using the indicated GMR-P[acman] construct as a donor for captured segment exchange. Only male progeny carrying the balancer CyO were scored for y and w phenotypes.
a
Two independent insertions of GMR-P[acman]-tsh50 and of GMR-P[acman]-tsh1 were tested.
b
Number of individual progeny in the F2 generation of the cross scheme in Figure 4 that had y− cuticle and w− eyes, indicative of a completed exchange.
c
Percentage of all F2 flies scored that had y− cuticle and w− eyes.