Figure 1 .

Dia2 is required for checkpoint recovery from MMS-induced DNA damage in S-phase. (A) Cells were arrested in late G1 by α-factor (αF), released into rich media (YPD) + 0.033% MMS for 40 min, and then released into YPD. Samples were analyzed at the indicated time points by flow cytometry. 1C and 2C indicate DNA content. Percentage of cells with 2C DNA content is indicated on the right of selected profiles. (B) Cells were arrested in late G1 by αF and then released into YPD at 30°. Samples were analyzed by flow cytometry. (C and D) DIA2 genetically interacts with Rad53-phosphatase PPH3 in response to MMS. (C) Tenfold serial dilutions of the indicated strains were spotted on YPD or YPD + 0.007% MMS and incubated at 30°. (D) Equal numbers of cells were plated on media containing the indicated amounts of MMS, and colony-forming units were counted after 4 days at 30°. Error bars represent standard deviations from three independent experiments. (E and F) Dia2 functions in parallel to Pph3 for S-phase checkpoint recovery. Samples were prepared and analyzed as described in A and B.