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. 1973 Dec;12(6):1401–1406. doi: 10.1128/jvi.12.6.1401-1406.1973

Host Effect on Arbovirus Replication: Appearance of Defective Interfering Particles in Murine Cells

Judith G Levin 1,2,1, Janet M Ramseur 1,2, Philip M Grimley 1,2
PMCID: PMC356781  PMID: 4796899

Abstract

Serial passage of Semliki Forest virus (SFV) in chicken embryo cells had little effect on SFV yield; however, high multiplicity infection of murine cells with one of the late passage pools (passage 9 SFV) resulted in a virus yield 10- to 20-fold lower than that obtained with earlier passage virus and 80-fold lower than the corresponding yield in chicken cells. This effect was accompanied by a striking decrease in the levels of 42S and 26S RNA and by increased proportions of a small single-stranded viral RNA (molecular weight, 9 × 105) and of a low-molecular-weight replicative form. There was also a reduction in the number of specific membranous structures previously associated with the group A arbovirus replication complex. These results suggested that passage 9 SFV contained defective interfering particles which were detected more readily after one passage in a murine indicator host cell. Identical results were obtained with two different murine cell lines: one a leukemia virus-free clone of AKR cells and the other JLS-V9 cells chronically infected with Rauscher leukemia virus. Host production of RNA tumor virus particles apparently did not affect arbovirus replication.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Choppin P. W. Replication of influenza virus in a continuous cell line: high yield of infective virus from cells inoculated at high multiplicity. Virology. 1969 Sep;39(1):130–134. doi: 10.1016/0042-6822(69)90354-7. [DOI] [PubMed] [Google Scholar]
  2. Eaton B. T., Faulkner P. Altered pattern of viral RNA synthesis in cells infected with standard and defective Sindbis virus. Virology. 1973 Jan;51(1):85–93. doi: 10.1016/0042-6822(73)90368-1. [DOI] [PubMed] [Google Scholar]
  3. Friedman R. M., Berezesky I. K. Cytoplasmic fractions associated with Semliki Forest virus ribonucleic acid replication. J Virol. 1967 Apr;1(2):374–383. doi: 10.1128/jvi.1.2.374-383.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Friedman R. M., Levin J. G., Grimley P. M., Berezesky I. K. Membrane-associated replication complex in arbovirus infection. J Virol. 1972 Sep;10(3):504–515. doi: 10.1128/jvi.10.3.504-515.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Friedman R. M., Levy H. B., Carter W. B. Replication of semliki forest virus: three forms of viral RNA produced during infection. Proc Natl Acad Sci U S A. 1966 Aug;56(2):440–446. doi: 10.1073/pnas.56.2.440. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Friedman R. M. Protein synthesis directed by an arbovirus. J Virol. 1968 Jan;2(1):26–32. doi: 10.1128/jvi.2.1.26-32.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Friedman R. M. Structural and nonstructural proteins of an arbovirus. J Virol. 1968 Oct;2(10):1076–1080. doi: 10.1128/jvi.2.10.1076-1080.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Grimley P. M., Berezesky I. K., Friedman R. M. Cytoplasmic structures associated with an arbovirus infection: loci of viral ribonucleic acid synthesis. J Virol. 1968 Nov;2(11):1326–1338. doi: 10.1128/jvi.2.11.1326-1338.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Grimley P. M., Levin J. G., Berezesky I. K., Friedman R. M. Specific membranous structures associated with the replication of group A arboviruses. J Virol. 1972 Sep;10(3):492–503. doi: 10.1128/jvi.10.3.492-503.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Huang A. S., Baltimore D. Defective viral particles and viral disease processes. Nature. 1970 Apr 25;226(5243):325–327. doi: 10.1038/226325a0. [DOI] [PubMed] [Google Scholar]
  11. Huang A. S., Wagner R. R. Defective T particles of vesicular stomatitis virus. II. Biologic role in homologous interference. Virology. 1966 Oct;30(2):173–181. doi: 10.1016/0042-6822(66)90093-6. [DOI] [PubMed] [Google Scholar]
  12. Kingsbury D. W., Portner A. On the genesis of incomplete Sendai virions. Virology. 1970 Dec;42(4):872–879. doi: 10.1016/0042-6822(70)90336-3. [DOI] [PubMed] [Google Scholar]
  13. Levin J. G., Friedman R. M. Analysis of arbovirus ribonucleic acid forms by polyacrylamide gel electrophoresis. J Virol. 1971 Apr;7(4):504–514. doi: 10.1128/jvi.7.4.504-514.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Martin E. M., Sonnabend J. A. Ribonucleic acid polymerase catalyzing synthesis of double-stranded arbovirus ribonucleic acid. J Virol. 1967 Feb;1(1):97–109. doi: 10.1128/jvi.1.1.97-109.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Perrault J., Holland J. Variability of vesicular stomatitis virus autointerference with different host cells and virus serotypes. Virology. 1972 Oct;50(1):148–158. doi: 10.1016/0042-6822(72)90355-8. [DOI] [PubMed] [Google Scholar]
  16. Rowe W. P., Hartley J. W., Lander M. R., Pugh W. E., Teich N. Noninfectious AKR mouse embryo cell lines in which each cell has the capacity to be activated to produce infectious murine leukemia virus. Virology. 1971 Dec;46(3):866–876. doi: 10.1016/0042-6822(71)90087-0. [DOI] [PubMed] [Google Scholar]
  17. Schlesinger S., Schlesinger M., Burge B. W. Defective virus particles from Sindbis virus. Virology. 1972 May;48(2):615–617. doi: 10.1016/0042-6822(72)90076-1. [DOI] [PubMed] [Google Scholar]
  18. Shenk T. E., Stollar V. Viral RNA species in BHK-21 cells infected with Sindbis virus serially passaged at high multiplicity of infection. Biochem Biophys Res Commun. 1972 Oct 6;49(1):60–67. doi: 10.1016/0006-291x(72)90009-5. [DOI] [PubMed] [Google Scholar]
  19. Sonnabend J. A., Martin E. M., Mécs E. Viral specific RNAs in infected cells. Nature. 1967 Jan 28;213(5074):365–367. doi: 10.1038/213365a0. [DOI] [PubMed] [Google Scholar]
  20. Stampfer M., Baltimore D., Huang A. S. Ribonucleic acid synthesis of vesicular stomatitis virus. I. Species of ribonucleic acid found in Chinese hamster ovary cells infected with plaque-forming and defective particles. J Virol. 1969 Aug;4(2):154–161. doi: 10.1128/jvi.4.2.154-161.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. TAYLOR J. STUDIES ON THE MECHANISM OF ACTION OF INTERFERON. I. INTERFERON ACTION AND RNA SYNTHESIS IN CHICK EMBRYO FIBROBLASTS INFECTED WITH SEMLIKI FOREST VIRUS. Virology. 1965 Mar;25:340–349. doi: 10.1016/0042-6822(65)90053-x. [DOI] [PubMed] [Google Scholar]
  22. Wright B. S., O'Brien P. A., Shibley G. P., Mayyasi S. A., Lasfargues J. C. Infection of an established mouse bone marrow cell line (JLS-V9) with Rauscher and Moloney murine leukemia viruses. Cancer Res. 1967 Sep;27(9):1672–1677. [PubMed] [Google Scholar]

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