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. 2012 Oct 23;23(2):290–299. doi: 10.1038/cr.2012.146

Figure 3.

Figure 3

Cytokinin receptors regulate PC interdigitation. (A) Representative PC images of 2-DAG cotyledon PC shapes in Col 0 and different cytokinin receptor mutants. (B) Quantitative analysis of lobe number per area (mean ± SEM) in (A). Asterisks indicate significant differences from Col 0 control (P < 0.001, t test). (C) Representative images of 2-DAG cotyledon PC shapes in wild type (WT) and the ahk3 cre1 cytokinin receptor mutant both with and without BA treatment. Bars = 20 μm. (D) Quantitative analysis of lobe number per area (mean ± SEM) in the treatments described in (C). Asterisks indicate a significant differences from untreated seedlings and WT (P <0.001, t test). The mean lobe number of ahk3 cre1 PCs is 9.8×10−3/μm2, whereas WT PCs at the same stage have a mean lobe number of 4.7×10−3/μm2. (E) RT-PCR analysis of AHK3 transcript level in two over-expression AHK3 lines: AHK3 OX-1 and AHK3 OX-2. (F) The representative PC images of 2-DAG AHK3 OX-1 cotyledons compared with Col 0. Bars = 20 μm. (G) Quantitative analysis of lobe number per area (mean ± SEM) in (F). Asterisks indicate significant differences from Col 0 seedlings (P < 0.001, t test).