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. 2012 Sep 18;23(2):242–253. doi: 10.1038/cr.2012.135

Figure 3.

Figure 3

Polymorphism CBS −4673C>G is related with CBS gene promoter hypomethylation. (A) After blocking DNA methylation in HEK293 cells using 5-Aza, CBS mRNA increased significantly compared with the control group. The actual value of 5-Aza group is 2.02 ± 0.22, and value of control group is 1.02 ± 0.19. Each value represents the mean ± SD of three experiments, and each experiment was performed in triplicate. (B) The methylation pattern of 20 CpG sites around the −4673 polymorphism (from −4 803 to −4 516) in 28 cardiovascular tissue samples. (C) Polymorphism CBS −4673C>G was related with allelic-specific methylation. (D) Nearly 70% of C alleles had at least one CpG site methylated, but almost 80% G alleles were not methylated at any CpG sites.