Fig. 3.

Levels of mRNA expression of individual conotoxins and conotoxin superfamilies in the venom duct of C. marmoreus. A, The total number of reads is plotted per precursor, demonstrating the efficacy of the sequencing effort. B, Dramatic variations in the level of expression were noted for individual conotoxin. Interestingly, most conotoxin with a number of reads above 300 were already discovered either from the venom or using PCR amplification strategies. C, The number of isoforms and the total number of reads per gene superfamily show an apparent correlation. The goodness of fit was R² = 0.88, revealing a significant correlation between the two parameters.