Table III. Conopeptide diversity: example of MrIA (Triple TOF data).
| Intensity precursor | Relative intensity | Precursor MW (Da) | Conf | Sequencea | Modifications | dMass (Da) |
|---|---|---|---|---|---|---|
| 9694 | 0.13 | 4535.26 | < 1 | VVVLPKTEDDVPMSSVYGNGKSILRGILRNGVCCGYKLCHPC | Oxidation(P)@41 | 0.0008 |
| 6240 | 0.08 | 4536.26 | < 1 | VVVLPKTEDDVPMSSVYGNGKSILRGILRNGVCCGYKLCHPC | Deamidated(N)@30; Oxidation(P)@41 | 0.0176 |
| 1540 | 0.02 | 2985.63 | 96.34 | VVVLPKTEDDVPMSSVYGNGKSILRGIL | 0.0053 | |
| 1267 | 0.02 | 2986.60 | 15.9 | VVVLPKTEDDVPMSSVYGNGKSILRGIL | Deamidated(N)@19 | −0.0007 |
| 25259 | 0.34 | 2703.43 | 99 | VVVLPKTEDDVPMSSVYGNGKSILR | Deamidated(N)@19 | 0.0153 |
| 20398 | 0.28 | 2702.44 | 99 | VVVLPKTEDDVPMSSVYGNGKSILR | 0.0108 | |
| 288699 | 3.90 | 2547.52 | 99 | VVVLPKTEDDVPMSSVYGNGKSIL | Deamidated(N)@19 | 0.2078 |
| 35465 | 0.48 | 2546.32 | 99 | VVVLPKTEDDVPMSSVYGNGKSIL | −0.0075 | |
| 4131 | 0.06 | 2433.25 | 19.5 | VVVLPKTEDDVPMSSVYGNGKSI | 0.0074 | |
| 2979 | 0.04 | 2434.24 | 46.5 | VVVLPKTEDDVPMSSVYGNGKSI | Deamidated(N)@19 | 0.0120 |
| 2590 | 0.04 | 2320.17 | 99 | VVVLPKTEDDVPMSSVYGNGKS | 0.0070 | |
| 1648 | 0.02 | 2321.16 | < 1 | VVVLPKTEDDVPMSSVYGNGKS | Deamidated(N)@19 | 0.0120 |
| 1996 | 0.03 | 2234.13 | 60.28 | VVVLPKTEDDVPMSSVYGNGK | Deamidated(N)@19 | 0.0132 |
| 2854 | 0.04 | 2105.03 | 99 | VVVLPKTEDDVPMSSVYGNG | −0.0035 | |
| 8022 | 0.11 | 1876.95 | 91.92 | VVVLPKTEDDVPMSSVY | −0.0001 | |
| 10443 | 0.14 | 1614.82 | 98.9 | VVVLPKTEDDVPMSS | 0.0020 | |
| 20565 | 0.28 | 1527.79 | 99 | VVVLPKTEDDVPMS | 0.0024 | |
| 214305 | 2.90 | 1440.85 | 99 | VVVLPKTEDDVPM | 0.0938 | |
| 4143 | 0.06 | 1456.75 | < 1 | VVVLPKTEDDVPM | Oxidation(M)@13 | −0.0001 |
| 2232 | 0.03 | 1309.72 | 67.7 | VVVLPKTEDDVP | 0.0068 | |
| 2321 | 0.03 | 998.56 | 13.6 | VVVLPKTED | −0.0009 | |
| 807 | 0.01 | 653.42 | 39.9 | VVVLPK | −0.0320 | |
| 7395262 | 100.00 | 1412.54 | 99 | NGVCCGYKLCHPC | Deamidated(N)@1; Oxidation(P)@12 | 0.0012 |
| 3680413 | 49.77 | 1411.55 | 99 | NGVCCGYKLCHPC | Oxidation(P)@12 | 0.0024 |
| 53999 | 0.73 | 1396.54 | 99 | NGVCCGYKLCHPC | Deamidated(N)@1 | 0.0001 |
| 37458 | 0.51 | 1395.54 | 99 | NGVCCGYKLCHPC | −0.0128 | |
| 28619 | 0.39 | 1394.53 | 99 | NGVCCGYKLCHPC | Amidated@C-term | −0.0422 |
| 6960 | 0.09 | 1309.53 | 72.2 | NGVCCGYKLCHP | Deamidated(N)@1; Oxidation(P)@12 | 0.0007 |
| 3680 | 0.05 | 1293.53 | 40.2 | NGVCCGYKLCHP | Deamidated(N)@1 | −0.0006 |
| 586 | 0.01 | 1371.59 | < 1 | NGVCCGYKLCHP | Sulfo(Y)@7; Amidated@C-term | 0.0748 |
| 24899 | 0.34 | 1059.42 | 99 | NGVCCGYKLC | Deamidated(N)@1 | −0.0011 |
| 8593 | 0.12 | 1058.44 | 99 | NGVCCGYKLC | 0.0053 | |
| 2577 | 0.03 | 956.41 | 99 | NGVCCGYKL | Deamidated(N)@1 | −0.0009 |
| 821 | 0.01 | 955.38 | 43.5 | NGVCCGYKL | Deamidated(N)@1; Amidated@C-term | −0.0445 |
| 1707 | 0.02 | 842.34 | 99 | NGVCCGYK | 0.0003 | |
| 23473 | 0.32 | 3899.82 | 19.6 | TEDDVPMSSVYGNGKSILRGILRNGVCCGYKLCHPC | Oxidation(M)@7 | −0.0078 |
| 15030 | 0.20 | 3900.81 | 52.7 | TEDDVPMSSVYGNGKSILRGILRNGVCCGYKLCHPC | Deamidated(N)@24; Oxidation(P)@35 | −0.0012 |
| 28026 | 0.38 | 2066.92 | 99 | TEDDVPMSSVYGNGKSILR | −0.0780 | |
| 2458 | 0.03 | 2067.99 | 8.2 | TEDDVPMSSVYGNGKSILR | Deamidated(N)@13 | 0.0075 |
| 178230 | 2.41 | 1910.89 | 99 | TEDDVPMSSVYGNGKSIL | −0.0049 | |
| 21181 | 0.29 | 1911.88 | 99 | TEDDVPMSSVYGNGKSIL | Deamidated(N)@13 | 0.0012 |
| 3141 | 0.04 | 1684.72 | 99 | TEDDVPMSSVYGNGKS | −0.0052 | |
| 7636 | 0.10 | 1469.60 | 63.07 | TEDDVPMSSVYGNG | −0.0010 | |
| 1397 | 0.02 | 1491.58 | 13.2 | TEDDVPMSSVYGN | Sulfo(Y)@11; Amidated@C-term | 0.0310 |
| 1752 | 0.02 | 1314.77 | < 1 | ILRGILRNGVCC | Amidated@C-term | 0.0316 |
| 228 | 0.00 | 956.60 | 95.9 | NGKSILRGI | 0.0271 | |
| 91329 | 1.23 | 1850.84 | 99 | GILRNGVCCGYKLCHPC | Oxidation(P)@19 | 0.0016 |
| 24507 | 0.33 | 1834.85 | < 1 | GILRNGVCCGYKLCHPC | 0.0032 | |
| 16702 | 0.23 | 1851.83 | 99 | GILRNGVCCGYKLCHPC | Deamidated(N)@5; Oxidation(P)@19 | 0.0056 |
| 67418 | 0.91 | 1810.79 | < 1 | GILRNGVCCGYKLCHP | Sulfo(Y)@11; Amidated@C-term | −0.0192 |
| 10658 | 0.14 | 1680.74 | 65.5 | LRNGVCCGYKLCHPC | Oxidation(P)@14 | 0.0005 |
| 17441 | 0.24 | 1294.52 | < 1 | RNGVCCGYKLC | Sulfo(Y)@8 | 0.0295 |
| 65392 | 0.88 | 1240.48 | 99 | VCCGYKLCHPC | Oxidation(P)@10 | −0.0026 |
| 1134 | 0.02 | 1205.34 | < 1 | CCGYKLCHPC | Sulfo(Y)@4 | −0.0358 |
| 15421 | 0.21 | 1038.41 | 99 | CGYKLCHPC | Oxidation(P)@8 | 0.0001 |
| 33291 | 0.45 | 999.27 | < 1 | GYKLCHPC | Sulfo(Y)@2 | −0.0932 |
| 2659 | 0.04 | 935.40 | 39.9 | GYKLCHPC | Oxidation(P)@7 | −0.0003 |
| 1195 | 0.02 | 878.38 | 9.4 | YKLCHPC | Oxidation(P)@6 | 0.0020 |
| 190945 | 2.58 | 1297.52 | 99 | GVCCGYKLCHPC | Oxidation(P)@11 | 0.0141 |
| 7964 | 0.11 | 1377.49 | 86.5 | GVCCGYKLCHPC | Sulfo(Y)@6; Oxidation(P)@11 | 0.0298 |
| 1106 | 0.01 | 1281.51 | < 1 | GVCCGYKLCHPC | −0.0035 | |
| 1940 | 0.03 | 2320.17 | 12.56 | PMSSVYGNGKSILRGILRNGVC | −0.0482 | |
| 1144 | 0.02 | 2292.17 | < 1 | PKTEDDVPMSSVYGNGKSILR | 0.0279 | |
| 1472 | 0.02 | 1821.79 | < 1 | PKTEDDVPMSSVYGNGK | Amidated@C-term | −0.0656 |
| 4293 | 0.06 | 1694.75 | 21 | PKTEDDVPMSSVYGNG | 0.0043 | |
| 2526 | 0.03 | 1717.62 | < 1 | PKTEDDVPMSSVYGN | Sulfo(Y)@13 | −0.0619 |
| 4231 | 0.06 | 1809.82 | 29.3 | EDDVPMSSVYGNGKSIL | −0.0225 | |
| 3515 | 0.05 | 1836.91 | < 1 | DDVPMSSVYGNGKSILR | 0.0023 | |
| 1243 | 0.02 | 1567.72 | 97.8 | DDVPMSSVYGNGKSI | 0.0050 | |
| 833 | 0.01 | 1454.64 | 97.9 | DDVPMSSVYGNGKS | 0.0011 | |
| 10443 | 0.14 | 1614.82 | 98.9 | VVLPKTEDDVPMSSV | 0.0020 | |
| 63416 | 0.86 | 1036.69 | < 1 | PGVVVLPKTE | Amidated@C-term | 0.0594 |
a Bold indicates peptide ion precursors with a relative intensity more than 1% of the deamidated MrIA.