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. 2012 Nov 21;12(2):381–394. doi: 10.1074/mcp.M112.019786

Fig. 3.

Fig. 3.

Silver stained 2D-PAGE (IEF) gels and respective 2D Western blots of (A) a representative primary breast tumor (T89), (B) MDA-MB-436 breast cancer cell line, and (C) purified recombinant full length Mage-A4 protein. A, Silver stained 2D IEF gel of proteins extracted from T89 (left hand panel). Protein spots 1 and 2 identified by mass spectrometry as Mage-A4 are indicated by black arrows. 2D Western blot of T89 developed with the anti-Mage-A4 mAb 57B antibody (right hand panel) matched the MS identities. B, Silver stained 2D IEF gel of proteins extracted from MDA-MB-436 cells. Protein spots 1–5 identified by mass spectrometry as Mage-A4 are indicated by black arrows (left hand panel). 2D Western blot of MDA-MB-436 cell line developed with the anti-Mage-A4 mAb 57B antibody (right-hand panel). C, 2D Western blot of purified recombinant full length Mage-A4 protein developed with the anti-Mage-A4 mAb 57B antibody.