Fig. 6.

Identification of Mage-A4 protein in tissue interstitial fluid of TNBC samples. A, 2D Western blot analyses of Mage-A4 confirmed externalization of Mage-A4 protein to the interstitial fluid of triple-negative tumor samples (TIF89). The pattern of expression was not completely identical to that of cellular lysates (compare DCTB T89 with TIF89, respectively), suggesting that only some of the forms of this protein are externalized. B, Differential expression of Mage-A4 was confirmed by IHC (antibody mAb 57B), showing that only tumor (DCTB T89; yellow arrow) but not normal cells express the protein. Scale bar, 100 μm. Additionally, full section image of low/high power IHC staining of TNBC tissue sample is presented in Supplemental Fig. S3.