Fig. 7.

Quantitative analysis of Mage-A4 protein by sandwich ELISA. A, Mage-A4 protein levels were determined in interstitial fluids from tumor samples, as well as available serum samples from our DCTB cohort by ELISA. One patient, DCTB 58, showed detectable levels of Mage-A4 protein in serum, as well as in TIF. Measurements were performed in triplicate and are expressed as mean values. Data shown are from a representative experiment out of three independent experiments. (B and C) Confirmation of Mage-A4 protein expression in DCTB patient 58. We confirmed the presence of Mage-A4 in tumor tissue and externalization of the protein by (B) 2D Western blots of tissue lysates (DCTB T58) and interstitial fluid (DCTB TIF58) of DCTB 58 tumor samples and (C) IHC (antibody mAb 57B). Scale bar, 50 μm.