Fig. 1.

The establishment and screen of antibody library. A, The immunized serum of #1 mouse showed the highest immunofluorescent intensity with lung cancer cells. B,The proliferation assay was performed with serum of #1 mice at 1:200 dilution. C, Left panel, the conditioned media of lung cancer cells (25 μg protein) were resolved on 9–15% gradient SDS gels and Coomassie stained. Right panel, the proteins in conditioned media were recognized by immunized serum using Western blot. D, Antigen recognized by mAb 13H3 was detected in four types of human lung cancer tissues and normal lung tissues (n = 40).