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. 2012 Nov 26;12(2):395–406. doi: 10.1074/mcp.M112.020784

Fig. 3.

Fig. 3.

Mac-2BP involved in proliferation in vitro and tumor growth in vivo. A, Proliferation assay of ANIP-973 and A549 cells. The 13H3 antibody significantly suppresses ANIP-973 and A549 cells proliferation in a dose-dependent manner. B, The effect of 13H3 treatment on the xenograft tumor growth. C, The expression of Mac-2BP expression on six lung cancer cell lines, including Glc-P, Glc-82, ANIP-973, A549, H1299 and H157. D, Semiquantitative reverse transcription-PCR was performed for amplification of Mac-2BP and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) using specific primers after siRNA transfection. Amplified bands were analyzed by agarose gel electrophoresis. Expression level of Mac-2BP was determined by densitometric analysis after normalizing RNA load. E, ANIP-973 and A549 cells transfected with Mac-2BP Si-RNA or NC control were seeded in 96-well plates for Cell Counting Kit-8 assay. The results are expressed as the mean optical density of absorbance of two independent experiments. F, EdU labeling showing proliferation of Mac-2BP silenced and control cells. The percentage of positive cells was derived from triplicate samples.