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. 2012 Nov 26;12(2):395–406. doi: 10.1074/mcp.M112.020784

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Fig. 4. — Induction of G1 arrest by down-regulation of Mac-2BP. A, The cell clones were cultured in complete medium for 48 h. Cells were then harvested, fixed with ethanol, stained with propidium iodide and analyzed by flow cytometry. The percentages of different cell cycle stages are shown in each panel. B, The levels of Mac-2BP, galectin-3, CDK2, CDK4, CDK6, CyclinD1, CyclinE, p21, p27 and were analyzed by Western blotting in A549 cells transfected with si-RNA-Mac-2 BP, or NC control. C, The levels of Mac-2BP, galectin-3, CDK2, CDK4, CDK6, CyclinD1, CyclinE, p21, p27 and were analyzed by Western blotting in ANIP-973 cells transfected with si-RNA-Mac-2 BP or NC control. D, The levels of CyclinD1, p27, and galectin-3 were analyzed by Western blotting in xenograft tumor treated with different dose of13H3 mAb.