Fig. 1.

LC-MS/MS identifies closely related Numb-interacting proteins form an endocytic complex: A, A schematic diagram showing the structure of the four Numb isoforms. 3XFLAG epitope tag sequences were added to either the amino or carboxyl terminal of Numb p72 or Numb 66 constructs, which were then used to generate stable cell lines. B, Visualized analysis of interactions between proteins identified in at least five of seven biologically distinct LC-MS/MS experiments with either Numb isoform, indicates that Numb-associated proteins are related, and together largely form part of an endocytic complex. Dotted edge lines represent newly identified Numb associations that have been confirmed by co-immunopurification and in vitro binding assays.