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. Author manuscript; available in PMC: 2013 Sep 6.
Published in final edited form as: Cancer Discov. 2012 Sep 6;2(9):798–811. doi: 10.1158/2159-8290.CD-12-0112

Figure 5. RAD51 foci formation (A) and modulation of proteins levels after PARP inhibitor treatment (B).

Figure 5

(A) Protein is localized at DNA DSBs region in response to stalled or collapsed DNA replication forks in SCLC (H69 & H82) but not in NSCLC cell (A549). Kinetics of RAD51 focus formation in NSCLC A549, SCLC H69, and SCLC H82. The percentage of cells with more than 5 nuclear foci was calculated. In each experiment, 100 nuclei were counted per data point. Error bars indicate standard error compared to unirradiated samples (*p<0.05). (B-C) Protein lysate was collected from three SCLC cell lines (H69, H82, H841) in duplicate at multiple timepoints (0-14d) after treatment with the PARP inhibitors AZD2281 and AGO14699. A time-dependent decrease was observed in multiple DNA repair proteins (B) and in other E2F1 targets such as thymidylate synthase (TS) and EZH2 (C). Note that TS follows a similar pattern to the other DNA repair proteins, while EZH2 is suppressed at 24h but recovers to baseline levels by 14d.