Table 1.
Effect of mutations in the liaIH operon on the expression of translational PliaI(opt)-gfp fusions
| Strain |
Relevant genotypea |
Promoter activity (fluorescence)b | |
|---|---|---|---|
| Expression plasmid | Strain background | ||
| TMB408 |
amyE:: pSJ5101 (PliaI-gfp) |
(WT168) PliaIliaIHTerm+ |
264 |
| TMB1172 |
amyE:: pLIKE-int+gfp |
(WT168) PliaIliaIHTerm+ |
1440 |
| TMB1174 |
(TMB604) ∆PliaIliaIH |
958 |
|
| TMB1153 |
(TMB1151) ∆liaIH |
1080 |
|
| TMB1318 |
(TMB1152) ∆liaIHTerm |
1416 |
|
| TMB1176 |
pLIKE-rep+gfp | (WT168) PliaIliaIHTerm+ |
9570 |
| TMB1178 |
(TMB604) ∆PliaIliaIH |
3372 |
|
| TMB1342 |
(TMB1151) ∆liaIH |
10607 |
|
| TMB1343 | (TMB1152) ∆liaIHTerm | 10870 | |
a The terminator downstream of liaH is abbreviated “Term”, its presence is indicated by a “+”. b Promoter activities were calculated taking the derivative of the fluorescence divided by the OD600 (dGFP/dt/OD600) at each time point.