Table 3.
qRT PCR validation of microarray results
| Schematic Name | Microarray Result | qRT-PCR-Result | Target Gene |
|---|---|---|---|
|
Redox Activity and Metalloenzyme Activity | |||
| contig 816 |
2.7 increase |
2.6 increase |
GSPD-816 (glyceraldehyde 3-phosphate dehydrogenase, cytosolic) |
| contig 3096 |
2.3 increase |
2.6 increase |
ASP (peroxisome type ascorbate peroxidase) |
| contig 119 |
2.7 increase |
2.3 increase |
CSD2 (copper/zinc dismutase 2) |
|
Metalloenzyme Activity | |||
| contig 180 |
1.95 increase |
3.5 increase |
MET (metallothionein) |
|
Vitamin C Biosynthesis and Antioxidant Activity | |||
| contig 3096 |
2.3 increase |
2.6 increase |
ASP (ascorbate peroxidase, peroxisome type) |
| contig 119 |
2.7 increase |
2.3 increase |
CSD2 (copper/zinc dismutase 2) |
| contig 3227 |
1.5 increase |
2.1 increase |
GPP (L-galactose-1-phosphate phosphatase) |
|
Organic Acid Metabolism | |||
| contig 816 |
2.7 increase |
2.6 increase |
GSPD-816 (glyceraldehyde 3-phosphate dehydrogenase, cytosolic) |
| contig 604 |
8.5 increase |
5.0 increase |
MGS (monogalactosyldiacylglycerol synthase) |
|
Stress Related | |||
| contig 35 |
6.3 decrease |
4.5 decrease |
GAST (GAST-like protein) |
|
Cysteine-Type Peptidase and Ubiquitin Proteolysis | |||
| contig 122 |
31.7 decrease |
20.6 decrease |
Ac-122 (Fruit Bromelain Ac-122) |
| contig 756 |
4.5 decrease |
2.0 increase |
OTU (OTU-like cysteine protease family protein) |
| contig 1299 | 3.1 increase | 2.1 increase | UBE2A (Ubiquitin conjugating protein) |